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Corbin, J.C.* ; Mensah, A.A.* ; Pieber, S.M.* ; Orasche, J. ; Michalke, B. ; Zanatta, M.* ; Czech, H.* ; Massabò, D.* ; Buatier De Mongeot, F.* ; Mennucci, C.* ; El Haddad, I.* ; Kumar, N.K.* ; Stengel, B.* ; Huang, Y.* ; Zimmermann, R. ; Prévôt, A.S.H.* ; Gysel, M.*

Trace metals in soot and PM2.5 from heavy-fuel-oil combustion in a marine engine.

Environ. Sci. Technol. 52, 6714-6722 (2018)
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Skin affections after sulfur mustard (SM) exposure include erythema, blister formation and severe inflammation. An antidote or specific therapy does not exist. Anti-inflammatory compounds as well as substances counteracting SM-induced cell death are under investigation. In this study, we investigated the benzylisoquinoline alkaloide berberine (BER), a metabolite in plants like berberis vulgaris, which is used as herbal pharmaceutical in Asian countries, against SM toxicity using a well-established in vitro approach. Keratinocyte (HaCaT) mono-cultures (MoC) or HaCaT/THP-1 co-cultures (CoC) were challenged with 100, 200 or 300 mM SM for 1 h. Post-exposure, both MoC and CoC were treated with 10, 30 or 50 mu M BER for 24 h. At that time, supernatants were collected and analyzed both for interleukine (IL) 6 and 8 levels and for content of adenylate-kinase (AK) as surrogate marker for cell necrosis. Cells were lysed and nucleosome formation as marker for late apoptosis was assessed. In parallel, AK in cells was determined for normalization purposes. BER treatment did not influence necrosis, but significantly decreased apoptosis. Anti-inflammatory effects were moderate, but also significant, primarily in CoC. Overall, BER has protective effects against SM toxicity in vitro. Whether this holds true should be evaluated in future in vivo studies.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cell-line Thp-1; In-vitro; Hacat Keratinocytes; Alkaloid Berberine; Dendritic Cells; Injury; Skin; Sensitization; Expression; Toxicity
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 0013-936X
e-ISSN 1520-5851
Journal Environmental Science & Technology
Quellenangaben Volume: 52, Issue: 11, Pages: 6714-6722 Article Number: , Supplement: ,
Publisher ACS
Publishing Place Washington, DC
Reviewing status Peer reviewed
Institute(s) Cooperation Group Comprehensive Molecular Analytics (CMA)
Research Unit BioGeoChemistry and Analytics (BGC)
POF-Topic(s) 30202 - Environmental Health
Research field(s) Environmental Sciences
PSP Element(s) G-504500-001
G-504800-002
DOI 10.1021/acs.est.8b01764
WOS ID WOS:000434892900068
Scopus ID 85048400434
Erfassungsdatum 2018-07-03
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