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Nemec, C.M.* ; Singh, A.K.* ; Ali, A.* ; Tseng, S.C.* ; Syal, K.* ; Ringelberg, K.J.* ; Ho, Y.H.* ; Hintermair, C. ; Ahmad, M.F.* ; Kar, R.K.* ; Gasch, A.P.* ; Akhtar, M.S.* ; Eick, D. ; Ansari, A.Z.*

Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner.

Nat. Chem. Biol. 15, 123–131 (2019)
Postprint DOI PMC
Open Access Green
Phosphorylation of the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) governs stage-specific interactions with different cellular machines. The CTD consists of Y1S2P3T4S5P6S7 heptad repeats and sequential phosphorylations of Ser7, Ser5 and Ser2 occur universally at Pol II-transcribed genes. Phosphorylation of Thr4, however, appears to selectively modulate transcription of specific classes of genes. Here, we identify ten new Thr4 kinases from different kinase structural groups. Irreversible chemical inhibition of the most active Thr4 kinase, Hrr25, reveals a novel role for this kinase in transcription termination of specific class of noncoding snoRNA genes. Genome-wide profiles of Hrr25 reveal a selective enrichment at 3' regions of noncoding genes that display termination defects. Importantly, phospho-Thr4 marks placed by Hrr25 are recognized by Rtt103, a key component of the termination machinery. Our results suggest that these uncommon CTD kinases place phospho-Thr4 marks to regulate expression of targeted genes.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Carboxy-terminal Domain; Transcription Elongation; Expression Programs; Genomic Expression; Capping Enzyme; Budding Yeast; Bur1 Kinase; Phosphorylation; Threonine-4; Distinct
Language
Publication Year 2019
Prepublished in Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 1552-4450
e-ISSN 1552-4469
Journal Nature Chemical Biology
Quellenangaben Volume: 15, Issue: 2, Pages: 123–131 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place Basingstoke
Reviewing status Peer reviewed
Institute(s) Institute of Functional Epigenetics (IFE)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Helmholtz Diabetes Center
PSP Element(s) G-502890-001
DOI 10.1038/s41589-018-0194-1
WOS ID WOS:000456188100011
Scopus ID 85059476046
PubMed ID 30598543
Erfassungsdatum 2018-12-31
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