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Metabolite analytics with integrated protein sensor on a microfluidic chip.
In: (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 27-31 October 2013, Freiburg). 2013. 868-870
Advances in metabolomics rely on quantification, investigation, optimization and streamlining new analytical appli- cations for small biomolecules. In this work a microfluidic large-scale integration chip pl atform for the characterization and use of biological proteins as sensors for metabolites is de veloped. Representatively, we selected the bacterial tran- scriptional regulator for the pyruvate dehydrogenase gene (PdhR) to tailor a synthetic assay for the metabolite pyruvate on chip [1]. PdhR belongs to a large family of transcriptional regulators, which either bind or dissociate from the DNA recognition sequence in dependence of cellular metabolite concentration. In here, this thermodynamic linked binding property of PdhR is exploited to measure pyruvate concentrations in high parallel fashion.
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Publication type
Article: Conference contribution
Keywords
Large-scale integration microfluidics, metabolic biosensor, synthetic biology, protein binding
thermodynamics
Conference Title
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Conference Date
27-31 October 2013
Conference Location
Freiburg
Quellenangaben
Pages: 868-870
Institute(s)
Helmholtz Pioneer Campus (HPC)