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Schneider, A.* ; Kurz, S.* ; Manske, K.* ; Janas, M.K.* ; Heikenwälder, M.* ; Misgeld, T.* ; Aichler, M. ; Weissmann, S.F.* ; Zischka, H. ; Knolle, P.* ; Wohlleber, D.*

Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.

Sci. Rep. 9:8492 (2019)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Flow-cytometry; Cultured-cells; Protein; Mechanisms; Mitophagy; Dynamics; Immunity; Disease; Model; Mavs
Language english
Publication Year 2019
HGF-reported in Year 2019
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 9, Issue: 1, Pages: , Article Number: 8492 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical Pathology (AAP)
Institute of Molecular Toxicology and Pharmacology (TOX)
POF-Topic(s) 30205 - Bioengineering and Digital Health
30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-500390-001
G-505200-003
DOI 10.1038/s41598-019-44922-9
WOS ID WOS:000470962500003
Scopus ID 85067056296
PubMed ID 31186476
Erfassungsdatum 2019-06-14
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