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Giopanou, I.* ; Kanellakis, N.I.* ; Giannou, A.D.* ; Lilis, I.* ; Marazioti, A.* ; Spella, M.* ; Papaleonidopoulos, V.* ; Simoes, D.C.M.* ; Zazara, D.E.* ; Agalioti, T.* ; Moschos, C.* ; Magkouta, S.* ; Kalomenidis, I.* ; Panoutsakopoulou, V.* ; Lamort, A.-S. ; Stathopoulos, G.T.

Osteopontin drives KRAS-mutant lung adenocarcinoma.

Carcinogenesis 41, 1134-1144 (2019)
Publ. Version/Full Text Postprint Research data DOI PMC
Open Access Green
Increased expression of osteopontin (secreted phosphoprotein 1, SPP1) is associated with aggressive human lung adenocarcinoma (LADC), but its function remains unknown. Our aim was to determine the role of SPP1 in smoking-induced LADC. We combined mouse models of tobacco carcinogen-induced LADC, of deficiency of endogenous Spp1 alleles, and of adoptive pulmonary macrophage reconstitution to map the expression of SPP1 and its receptors and determine its impact during carcinogenesis. Co-expression of Spp1 and mutant Kras(G12C) in benign cells was employed to investigate SPP1/KRAS interactions in oncogenesis. Finally, intratracheal adenovirus encoding Cre recombinase was delivered to LSL.KRAS(G12D) mice lacking endogenous or overexpressing transgenic Spp1 alleles. SPP1 was overexpressed in experimental and human LADC and portended poor survival. In response to two different smoke carcinogens, Spp1-deficient mice developed fewer and smaller LADC with decreased cellular survival and angiogenesis. Both lung epithelial- and macrophage-secreted SPP1 drove tumor-associated inflammation, while epithelial SPP1 promoted early tumorigenesis by fostering the survival of KRAS-mutated cells. Finally, loss and overexpression of Spp1 was, respectively, protective and deleterious for mice harboring KRAS(G12D)-driven LADC. Our data support that SPP1 is functionally involved in early stages of airway epithelial carcinogenesis driven by smoking and mutant KRAS and may present an important therapeutic target.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Kras ; Spp1 ; Lung Cancer ; Survival ; Urethane; Early-stage; Cancer; Promotes; Expression; Resistance; Cells; Tumorigenesis; Progression; Activation; Invasion
ISSN (print) / ISBN 0143-3334
e-ISSN 1460-2180
Journal Carcinogenesis
Quellenangaben Volume: 41, Issue: 8, Pages: 1134-1144 Article Number: , Supplement: ,
Publisher Oxford University Press
Publishing Place Great Clarendon St, Oxford Ox2 6dp, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Lung Health and Immunity (LHI)
Grants REPSIRE2 European Respiratory Society Fellowship
Greek national funds through an action entitled "Reinforcement of Postdoctoral Researchers" (NSRF 2014 -2020)
Greek State Scholarship Foundation (IKY) programme - European Union (European Social Fund-ESF)
European Research Council
Hellenic Thoracic Society