Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text Volltext
DOI
PMC
 |
Free by publisher |
Open Access Green as soon as Postprint is submitted to ZB.
In vivo cell biology using Gal4-mediated multicolor subcellular labeling in zebrafish.
Commun. Integr. Biol. 4, 336-339 (2011)
Publ. Version/Full Text Volltext
DOI
PMC
The behavior of a cell is determined by the interplay of its subcellular components. Thus, being able to simultaneously visualize several organelles inside cells within the natural context of a living organism could greatly enhance our understanding of developmental processes. We have established a Gal4-based system for the simultaneous and cell type specific expression of multiple subcellular labels in transparent zebrafish embryos. This system offers the opportunity to follow intracellular developmental processes in a live vertebrate organism using confocal fluorescence time-lapse microscopy. Using this approach we recently showed that the centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in the zebrafish cerebellum in vivo. Here we present additional in vivo findings about the centrosomal and microtubule dynamics of neuroepithelial cells during mitotic cleavages at early neural tube stages.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
in vivo imaging; zebrafish; neuroepithelium; Gal4/AUS; subcellular labeling; mitosis
e-ISSN
1942-0889
Quellenangaben
Volume: 4,
Issue: 3,
Pages: 336-339
Publisher
Landes Bioscience
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)