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Kjell, J. ; Fischer-Sternjak, J. ; Thompson, A.J.* ; Friess, C.* ; Sticco, M.J.* ; Salinas, F.* ; Cox, J.* ; Martinelli, D.C.* ; Ninkovic, J. ; Franze, K.* ; Schiller, H. B. ; Götz, M.

Defining the adult neural stem cell niche proteome identifies key regulators of adult neurogenesis.

Cell Stem Cell 26, 277-293.e8 (2020)

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	Open Access Gold (Paid Option)

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The mammalian brain contains few niches for neural stem cells (NSCs) capable of generating new neurons, whereas other regions are primarily gliogenic. Here we leverage the spatial separation of the sub-ependymal zone NSC niche and the olfactory bulb, the region to which newly generated neurons from the sub-ependymal zone migrate and integrate, and present a comprehensive proteomic characterization of these regions in comparison to the cerebral cortex, which is not conducive to neurogenesis and integration of new neurons. We find differing compositions of regulatory extracellular matrix (ECM) components in the neurogenic niche. We further show that quiescent NSCs are the main source of their local ECM, including the multi-functional enzyme transglutaminase 2, which we show is crucial for neurogenesis. Atomic force microscopy corroborated indications from the proteomic analyses that neurogenic niches are significantly stiffer than non-neurogenic parenchyma. Together these findings provide a powerful resource for unraveling unique compositions of neurogenic niches.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords C1ql3 ; Cerebral Cortex ; Extracellular Matrix ; Neuroblast ; Olfactory Bulb ; S100a6 ; Subventricular Zone ; Tissue Stiffness ; Transglutaminase2 ; Transit-amplifying Progenitor; Mouse Subependymal Zone; Extracellular-matrix; Progenitor Cells; Neuronal Differentiation; Tenascin-c; Rat-brain; Migration; Lineage; Transplantation; Proliferation

ISSN (print) / ISBN 1934-5909

e-ISSN 1875-9777

Journal Cell Stem Cell

Quellenangaben Volume: 26, Issue: 2, Pages: 277-293.e8

Publisher Cell Press

Publishing Place Cambridge, Mass.

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Stem Cell Research (ISF)
Lung Health and Immunity (LHI)