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Prakash, J. ; Seyedebrahimi, M.M. ; Ghazaryan, A. ; Malekzadeh Najafabadi, J. ; Gujrati, V. ; Ntziachristos, V.

Short-wavelength optoacoustic spectroscopy based on water muting.

Proc. Natl. Acad. Sci. U.S.A. 117, 4007-4014 (2020)
Publ. Version/Full Text Postprint DOI PMC
Infrared (IR) optoacoustic spectroscopy can separate a multitude of molecules based on their absorption spectra. However, the technique is limited when measuring target molecules in aqueous solution by strong water absorption at IR wavelengths, which reduces detection sensitivity. Based on the dependence of optoacoustic signal on the temperature of the probed medium, we introduce cooled IR optoacoustic spectroscopy (CIROAS) to mute water contributions in optoacoustic spectroscopy. We showcase that spectral measurements of proteins, lipids, and glucose in the short-wavelength IR region, performed at 4 degrees C, lead to marked sensitivity improvements over conventional optoacoustic or IR spectroscopy. We elaborate on the dependence of optoacoustic signals on water temperature and demonstrate polarity changes in the recorded signal at temperatures below 4 degrees C. We further elucidate the dependence of the optoacoustic signal and the muting temperature on sample concentration and demonstrate that changes in these dependences enable quantification of the solute concentration. We discuss how CIROAS may enhance abilities for molecular sensing in the IR.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Sensing ; Photoacoustics ; Near Infrared ; Temperature ; Spectroscopy; Photoacoustic-spectroscopy; Blood-glucose; Real-time; Temperature; Tomography; Brain; Density; Maximum
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Journal Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Volume: 117, Issue: 8, Pages: 4007-4014 Article Number: , Supplement: ,
Publisher National Academy of Sciences
Publishing Place 2101 Constitution Ave Nw, Washington, Dc 20418 Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)