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He, X.* ; Reichl, F.X.* ; Milz, S.* ; Michalke, B. ; Wu, X. ; Sprecher, C.M.* ; Yang, Y.* ; Gahlert, M.* ; Röhling, S.* ; Kniha, H.* ; Hickel, R.* ; Högg, C.*

Titanium and zirconium release from titanium- and zirconia implants in mini pig maxillae and their toxicity in vitro.

Dent. Mater. 36, 402-412 (2020)

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OBJECTIVE: Titanium (Ti)- and Zirconia (ZrO₂)-implants in mini pig maxillae were compared with respect to Ti/zirconium (Zr) release into the surrounding bone tissues, the resulting short term tissue responses and the potential toxicity. METHODS: Ti/Zr release from Ti- and ZrO₂-implants in mini pig maxillae was determined with inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). The spatial distribution of Ti and Zr in maxilla tissues near the implant surface was assessed with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). A histological analysis was performed to investigate the tissue responses after 12 weeks of implantation. The cytotoxicity and DNA damage of Ti particles and ZrO₂ particles were studied with XTT and Comet assay. RESULTS: The mean Ti content in the bone adjacent to Ti-implants was 1.67 mg/kg-bone weight. The highest Ti content detected was 2.17 mg/kg-bone weight. The mean Zr content in the bone adjected to ZrO₂-implants was 0.59 mg/kg-bone weight. The highest Zr content was 0.75 mg/kg-bone weight. The spatial distribution of the Ti and Zr in bone showed mainly a higher intensity of Ti and Zr close to the screw thread outer tip rather. Histological analysis indicated that near both implant-types signs of bone marrow fibrosis were present. EC₅₀ of commercially available ZrO₂-nanoparticles (NPs, <100 nm) and ZrO₂-microparticles (MPs, <5 μm) was 13.96 mg/ml and 80.99 mg/ml, respectively. ZrO₂-NPs and ZrO₂-MPs can induce DNA damage at 70 μg/ml and 810 μg/ml, respectively. SIGNIFICANCE: After 12-weeks of implantation, increased concentrations of Ti and Zr can be detected in bone/tissues near Ti- and ZrO₂-implants in mini pig maxillae. Ti content released from Ti-implants is two times higher than the Zr content released from ZrO₂-implants. ZrO₂-NPs showed lower cytotoxicity and DNA damage compared to results reported for Ti-NPs in human cells.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Bone Tissue ; Dna Damage ; Icp-ms ; Icp-oes ; La-icp-ms ; Nanoparticles ; Pdl Cells ; Sem ; Sla ; Xtt

ISSN (print) / ISBN 0109-5641

e-ISSN 0109-5641

Journal Dental Materials

Quellenangaben Volume: 36, Issue: 3, Pages: 402-412

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Analytical BioGeoChemistry (BGC)
Cooperation Group Comprehensive Molecular Analytics (CMA)