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Timsy, T.* ; Spanner, T.* ; Ulrich, A.* ; Kublik, S. ; Fösel, B. ; Kolb, S.* ; Horn, M.A.* ; Behrendt, U.*

Pseudomonas campi sp. Nov., a nitrate-reducing bacterium isolated from grassland soil.

Int. J. Syst. Evol. Microbiol. 71, DOI: 10.1099/ijsem.0.004799 (2021)
Postprint DOI PMC
Open Access Green
A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2 revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2 =LMG 31521 =DSM 110222 ) is proposed. T T T T
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Publication type Article: Journal article
Document type Scientific Article
Keywords Assimilative Nitrate Reduction ; Phylogenomic Analysis ; Polyphasic Taxonomy ; S: Pseudomonas Campi Sp. Nov ; Soil Bacterium; Reduction; Taxonomy; Grasses
Language english
Publication Year 2021
HGF-reported in Year 2021
ISSN (print) / ISBN 1466-5026
e-ISSN 1466-5034
Journal International Journal of Systematic and Evolutionary Microbiology
Quellenangaben Volume: 71, Issue: 5 Pages: , Article Number: , Supplement: ,
Publisher SGM
Publishing Place Reading
Reviewing status Peer reviewed
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)
POF-Topic(s) 30202 - Environmental Health
Research field(s) Environmental Sciences
PSP Element(s) G-504700-001
G-504700-003
Grants DFG Priority Program 1374 'Infrastructure-Biodiversity-Exploratories'
DOI 10.1099/ijsem.0.004799
WOS ID WOS:000651605900003
Scopus ID 85105189138
PubMed ID 34016249
Erfassungsdatum 2021-06-21
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