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Pseudomonas campi sp. Nov., a nitrate-reducing bacterium isolated from grassland soil.
Int. J. Syst. Evol. Microbiol. 71, DOI: 10.1099/ijsem.0.004799 (2021)
A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2 revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2 =LMG 31521 =DSM 110222 ) is proposed. T T T T
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Assimilative Nitrate Reduction ; Phylogenomic Analysis ; Polyphasic Taxonomy ; S: Pseudomonas Campi Sp. Nov ; Soil Bacterium; Reduction; Taxonomy; Grasses
ISSN (print) / ISBN
1466-5026
e-ISSN
1466-5034
Quellenangaben
Volume: 71,
Issue: 5
Publisher
SGM
Publishing Place
Reading
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Comparative Microbiome Analysis (COMI)
Grants
DFG Priority Program 1374 'Infrastructure-Biodiversity-Exploratories'