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Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo.
STAR Protoc. 2:100636 (2021)
We here report a flow-cytometry-based protocol to measure single-cell protein expression in small samples. The protocol is optimized for simultaneous detection of fluorescent proteins and intracellular and surface antigens in the embryonic pancreas from the mouse. Owing to low cell numbers, current protocols for flow cytometric analysis of embryonic tissues rely on tissue pooling. Our protocol enables analysis of one pancreas per sample, thereby facilitating detection of biological variation and minimizing the number of experimental animals needed. For complete details on the use and execution of this protocol, please refer to Nyeng et al (2019).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Isolation ; Developmental Biology ; Flow Cytometry/mass Cytometry ; Molecular Biology ; Single Cell
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 2,
Issue: 3,
Article Number: 100636
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Transl. Stem Cell Research (ITS)