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A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.
STAR Protoc. 2:100700 (2021)
With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cancer ; Cell Biology ; Microscopy ; Molecular Biology
Language
english
Publication Year
2021
HGF-reported in Year
2021
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 2,
Issue: 3,
Article Number: 100700
Reviewing status
Peer reviewed
Institute(s)
Institute of Diabetes and Cancer (IDC)
POF-Topic(s)
90000 - German Center for Diabetes Research
Research field(s)
Helmholtz Diabetes Center
PSP Element(s)
G-501900-251
PubMed ID
34401774
Erfassungsdatum
2021-10-04