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Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing.
STAR Protoc. 2:100805 (2021)
Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downstream sequencing analyses. The adaptation to frozen tissue provides a pause point that allows sampling at multiple time points and/or phenotypic characterization of livers prior to nuclei isolation and downstream analyses. For complete details on the use of this protocol, please refer to Loft et al. (2021).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Biology ; Cell Isolation ; Cell Separation/fractionation ; Genomics ; Molecular Biology ; Rnaseq ; Sequencing
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 2,
Issue: 3,
Article Number: 100805
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Diabetes and Cancer (IDC)