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Delineating chromosomal breakpoints in radiation-induced papillary thyroid cancer.
Genes 2, 397-419 (2011)
Recurrent translocations are well known hallmarks of many human solid tumors and hematological disorders, where patient- and breakpoint-specific information may facilitate prognostication and individualized therapy. In thyroid carcinomas, the proto-oncogenes RET and NTRK1 are often found to be activated through chromosomal rearrangements. However, many sporadic tumors and papillary thyroid carcinomas (PTCs) arising in patients with a history of exposure to elevated levels of ionizing irradiation do not carry these known abnormalities. We developed a rapid scheme to screen tumor cell metaphase spreads and identify candidate genes of tumorigenesis and neoplastic progression for subsequent functional studies. Using a series of overnight fluorescence in situ hybridization (FISH) experiments with pools comprised of bacterial artificial chromosome (BAC) clones, it now becomes possible to rapidly refine breakpoint maps and, within one week, progress from the low resolution Spectral Karyotyping (SKY) maps or Giemsa-banding (G-banding) karyotypes to fully integrated, high resolution physical maps including a list of candiate genes in the critical regions.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Chernobyl; neoplastic disease; papillary thyroid cancer; translocation; molecular cytogenetics; breakpoint delineation; fluorescence in situ hybridization; bacterial artificial chromosomes
ISSN (print) / ISBN
2073-4425
e-ISSN
2073-4425
Journal
Genes
Quellenangaben
Volume: 2,
Issue: 3,
Pages: 397-419
Publisher
MDPI
Non-patent literature
Publications
Reviewing status
Peer reviewed