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Rodrigues, E.M. ; Stiel, A.-C.

It's a two-way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes.

FEBS Lett. 597, 1319-1344 (2023)

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	Open Access Gold (Paid Option)

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Chromophore-bearing proteins that are (reversibly) altered after light illumination are major functional components of nature. They gained considerable attention in the last decades since the dynamic interactions of the chromophore and protein matrix can be used to control downstream effects altering the functionality of proteins, cells, or complete organisms with light (optogenetics). Additionally, the photophysical effects can be employed to add capabilities to optical imaging. For example, light can be used to reversibly switch the signal on or off (e.g., fluorescence). In this article, we review chromophore and protein matrix interactions, focusing on photoswitching fluorescent proteins of the GFP family (RSFPs) and natively photoswitching bacteriophytochromes (BphPs). This review aims to provide an in-depth understanding of the dynamic interplay between photoswitching photophysics and the protein matrix and a thorough discussion on how this connection has been harnessed for the development of optogenetic and imaging tools.

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Publication type Article: Journal article

Document type Review

Corresponding Author

Keywords Bacteriophytochromes ; Optical Imaging ; Optogenetics ; Photophysics ; Protein Engineering ; Reversibly Switchable Fluorescent Proteins ; Structural Studies; Cis-trans Isomerization; Bacterial Phytochrome; Structural Basis; Histidine Kinases; Crystal-structure; Optical Control; Excited-state; Chromophore; Mechanism; Reveals

ISSN (print) / ISBN 0014-5793

e-ISSN 1873-3468

Journal FEBS Letters

Quellenangaben Volume: 597, Issue: 10, Pages: 1319-1344

Publisher Elsevier

Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Biological and Medical Imaging (IBMI)

Grants Horizon Europe - Pillar III
European Research Council (ERC)
Projekt DEAL
Deutsche Forschungsgemeinschaft
European Union
European Research Council (ERC) under the European Union