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Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment.
Stem Cell Rep. 18, 1972-1986 (2023)
The formation of vascular structures is fundamental for in vitro tissue engineering. Vascularization can enable the nutrient supply within larger structures and increase transplantation efficiency. We differentiated human induced pluripotent stem cells toward endothelial cells in 3D suspension culture. To investigate in vitro neovascularization and various 3D microenvironmental approaches, we designed a comprehensive single-cell transcriptomic study. Time-resolved single-cell transcriptomics of the endothelial and co-evolving mural cells gave insights into cell type development, stability, and plasticity. Transfer to a 3D hydrogel microenvironment induced neovascularization and facilitated tracing of migrating, coalescing, and tubulogenic endothelial cell states. During maturation, we monitored two pericyte subtypes evolving mural cells. Profiling cell-cell interactions between pericytes and endothelial cells revealed angiogenic signals during tubulogenesis. In silico discovered ligands were tested for their capability to attract endothelial cells. Our data, analyses, and results provide an in vitro roadmap to guide vascularization in future tissue engineering.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Culturing Technologies ; Inferred Ec-pericyte Interactions ; Microfluidic Ligand Assay ; Neovascularization ; Single-cell Mrna Sequencing ; Stem Cell-derived Endothelial Cells; Growth-factor; Vegf; Contributes; Generation; Derivation; Regulator; Migration; Promotes; Cxcr4
ISSN (print) / ISBN
2213-6711
Journal
Stem Cell Reports
Quellenangaben
Volume: 18,
Issue: 10,
Pages: 1972-1986
Publisher
Cell Press
Publishing Place
Maryland Heights, MO
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Pioneer Campus (HPC)
Research Unit Analytical Pathology (AAP)
Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Research Unit Analytical Pathology (AAP)
Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Grants
BMBF - SprintD
Helmholtz Pioneer Campus
ERC
BMBF - SprintD
Helmholtz Pioneer Campus
ERC