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Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography.
Curr. Opin. Cell Biol. 88:102356 (2024)
Cryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic reconstructions and the serial lift-out sample preparation procedure will soon make it possible to perform high-resolution structural biology in a whole new range of samples, from multicellular organisms to organoids and tissues.
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Publication type
Article: Journal article
Document type
Review
Keywords
Actin Filaments ; Automation ; Cryo-electron Tomography ; Deep Learning-based Data Analysis ; Lift-out ; Molecular Sociology ; Tomogram Acquisition; Organization; Resolution
ISSN (print) / ISBN
0955-0674
e-ISSN
1879-0410
Journal
Current Opinion in Cell Biology
Quellenangaben
Volume: 88,
Article Number: 102356
Publisher
Elsevier
Publishing Place
Kidlington
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Pioneer Campus (HPC)
Grants
Deutsche Forschungsgemeinschaft (DFG)