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Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography.

Curr. Opin. Cell Biol. 88:102356 (2024)
Publ. Version/Full Text DOI PMC
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Cryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic reconstructions and the serial lift-out sample preparation procedure will soon make it possible to perform high-resolution structural biology in a whole new range of samples, from multicellular organisms to organoids and tissues.
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Publication type Article: Journal article
Document type Review
Keywords Actin Filaments ; Automation ; Cryo-electron Tomography ; Deep Learning-based Data Analysis ; Lift-out ; Molecular Sociology ; Tomogram Acquisition; Organization; Resolution
Language english
Publication Year 2024
HGF-reported in Year 2024
ISSN (print) / ISBN 0955-0674
e-ISSN 1879-0410
Quellenangaben Volume: 88, Issue: , Pages: , Article Number: 102356 Supplement: ,
Publisher Elsevier
Publishing Place Kidlington
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Pioneer Campus
PSP Element(s) G-510008-001
Grants Deutsche Forschungsgemeinschaft (DFG)
PubMed ID 38608425
Erfassungsdatum 2024-06-04