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Multimodal techniques and strategies for chemical and metabolic imaging at the single-cell level.
Curr. Opin. Biotechnol. 88:103149 (2024)
Single-cell chemical and metabolic imaging technologies provide unprecedented insights into individual cell dynamics, advancing our understanding of cellular processes, molecular interactions, and metabolic activities. Advances in fluorescence, Raman, optoacoustic (photoacoustic), or mass spectrometry methods have paved the way to characterize metabolites, signaling molecules, and other moieties within individual cells. These modalities can also lead to single-cell imaging capabilities by targeting endogenous cell contrast or by employing exogenous contrast generation techniques, including contrast agents that target specific cell structure or function. In this review, we present key developments, summarize recent applications in single-cell interrogation and imaging, and illustrate their advantages, limitations, and outlook.
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Publication type
Article: Journal article
Document type
Review
Keywords
Technologies; Microscopy
ISSN (print) / ISBN
0958-1669
e-ISSN
1879-0429
Journal
Current Opinion in Biotechnology
Quellenangaben
Volume: 88,
Article Number: 103149
Publisher
Elsevier
Publishing Place
Amsterdam
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)