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Selmi, H. ; Walker, A. ; Debarbieux, L.* ; Schmitt-Kopplin, P.

Improving the intestinal lipidome coverage in a gnotobiotic mouse model using UHPLC-MS-based approach through optimization of mobile phase modifiers and column selection.

J. Chromatogr. B 1242:124188 (2024)

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Lipidomics is focusing on the screening of lipid species in complex mixtures using mass spectrometry-based approaches. In this work, we aim to enhance the intestinal lipidome coverage within the Oligo-Mouse-Microbiota (OMM12) colonized mouse model by testing eight mobile phase conditions on five reversed-phase columns. Our selected mobile phase modifiers included two ammonium salts, two concentrations, and the addition of respective acids at 0.1 %. We compared two columns with hybrid surface technology, two with ethylene bridged hybrid technology and one with core–shell particles. Best performance was attained for standards and intestinal lipidome, using either ammonium formate or acetate in ESI(+) or ammonium acetate in ESI(−) for all column technologies. Notably, a concentration of 5 mM ammonium salt showed optimal results for both modes, while the addition of acids had a negligible effect on lipid ionization efficiency. The HST BEH C18 column improved peak width and tailing factor parameters compared to other technologies. We achieved the highest lipid count in colon and ileum content, including ceramides, phosphatidylethanolamines and phosphatidylcholines, when using 5 mM ammonium acetate in ESI(−). Conversely, in ESI(+) 5 mM ammonium formate demonstrated superior coverage for diacylglycerols and triacylglycerols.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Hybrid Surface Technology ; Mobile Phase Modifiers ; Non-targeted Lipidomics ; Oligo-mouse-microbiota; Mass-spectrometry; Chromatography; Plasma; Uplc; Performance; Homeostasis; Extraction

ISSN (print) / ISBN 1570-0232

e-ISSN 1873-376X

Journal Journal of Chromatography

Quellenangaben Volume: 1242, Article Number: 124188

Publisher Elsevier

Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Analytical BioGeoChemistry (BGC)

Grants DFG
PRCI