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Kohantorabi, M.* ; Ugolotti, A.* ; Sochor, B.* ; Rössler, J. ; Wagstaffe, M.* ; Meinhardt, A.* ; Beck, E.E.* ; Dolling, D.S.* ; Garcia, M.B.* ; Creutzburg, M.* ; Keller, T.F.* ; Schwartzkopf, M.* ; Vayalil, S.K.* ; Thuenauer, R.* ; Guédez, G.* ; Löw, C.* ; Ebert, G. ; Protzer, U. ; Hammerschmidt, W. ; Zeidler, R. ; Roth, S.V.* ; Di Valentin, C.* ; Stierle, A.* ; Noei, H.*

Light-induced transformation of virus-like particles on TiO2.

ACS Appl. Mater. Interfaces 16, 37275-37287 (2024)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Titanium dioxide (TiO2) shows significant potential as a self-cleaning material to inactivate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and prevent virus transmission. This study provides insights into the impact of UV-A light on the photocatalytic inactivation of adsorbed SARS-CoV-2 virus-like particles (VLPs) on a TiO2 surface at the molecular and atomic levels. X-ray photoelectron spectroscopy, combined with density functional theory calculations, reveals that spike proteins can adsorb on TiO2 predominantly via their amine and amide functional groups in their amino acids blocks. We employ atomic force microscopy and grazing-incidence small-angle X-ray scattering (GISAXS) to investigate the molecular-scale morphological changes during the inactivation of VLPs on TiO2 under light irradiation. Notably, in situ measurements reveal photoinduced morphological changes of VLPs, resulting in increased particle diameters. These results suggest that the denaturation of structural proteins induced by UV irradiation and oxidation of the virus structure through photocatalytic reactions can take place on the TiO2 surface. The in situ GISAXS measurements under an N2 atmosphere reveal that the virus morphology remains intact under UV light. This provides evidence that the presence of both oxygen and UV light is necessary to initiate photocatalytic reactions on the surface and subsequently inactivate the adsorbed viruses. The chemical insights into the virus inactivation process obtained in this study contribute significantly to the development of solid materials for the inactivation of enveloped viruses.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Afm ; Gisaxs ; Sars-cov-2 Virus-like Particles (vlps) ; Xps ; Photocatalytic Oxidation ; Titanium Dioxide; Protein Adsorption; Inactivation; Spectroscopy; Acids; Xps
ISSN (print) / ISBN 1944-8244
e-ISSN 1944-8252
Journal ACS applied materials & interfaces
Quellenangaben Volume: 16, Issue: 28, Pages: 37275-37287 Article Number: , Supplement: ,
Publisher ACS
Publishing Place Washington, DC
Non-patent literature Publications
Institute(s) Research Unit Gene Vector (AGV)
Institute of Virology (VIRO)
Grants European Union-NextGenerationEU through the Italian Ministry of University and Research
Initiative and networking fund of the Helmholtz Association of German Research Centers under the CORAERO Project