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Native ChIP: Studying the genome-wide distribution of histone modifications in cells and tissue.
In: Chromatin Immunoprecipitation. Berlin [u.a.]: Springer, 2024. 1-16 (Methods Mol. Biol. ; 2846)
For the genome-wide mapping of histone modifications, chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing remains the benchmark method. While crosslinked ChIP can be used for all kinds of targets, native ChIP is predominantly used for strong and direct DNA interactors like histones and their modifications. Here we describe a native ChIP protocol that can be used for cells and tissue material.
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Publication type
Article: Edited volume or book chapter
Keywords
Chip-seq ; Chromatin Immunoprecipitation (chip) ; Histone Modifications ; Native Protein-dna Interactions
Language
english
Publication Year
2024
HGF-reported in Year
2024
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Chromatin Immunoprecipitation
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2846,
Pages: 1-16
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Functional Epigenetics (IFE)
POF-Topic(s)
30203 - Molecular Targets and Therapies
Research field(s)
Helmholtz Diabetes Center
PSP Element(s)
G-502800-001
Scopus ID
85201242636
PubMed ID
39141226
Erfassungsdatum
2024-10-01