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Genomic analysis of Aeromonas salmonicida ssp. salmonicida isolates collected during multiple clinical outbreaks supports association with a Single epidemiological unit.
Pathogens 13:908 (2024)
Outbreaks of furunculosis cause significant losses in salmonid aquaculture worldwide. With a recent rise in antimicrobial resistance, regulatory measures to minimize the use of antibiotics in animal husbandry, including aquaculture, have increased scrutiny and availability of veterinary medical products to control this disease in production facilities. In such a regulatory environment, the utility of autogenous vaccines to assist with disease prevention and control as a veterinary-guided prophylactic measure is of high interest to the producers and veterinary services alike. However, evolving concepts of epidemiological units and epidemiological links need to be considered during approval and acceptance procedures for the application of autogenous vaccines in multiple aquaculture facilities. Here, we present the results of solid-state nanopore sequencing (Oxford Nanopore Technologies, ONT) performed on 54 isolates of Aeromonas salmonicida ssp. salmonicida sampled during clinical outbreaks of furunculosis in different aquaculture facilities from Bavaria, Germany, from 2017 to 2020. All of the performed analyses (phylogeny, single nucleotide polymorphism and 3D protein modeling for major immunogenic proteins) support a high probability that all studied isolates belong to the same epidemiological unit. Simultaneously, we describe a cost/effective method of whole genome analysis with the usage of ONT as a viable strategy to study outbreaks of other pathogens in the field of aquatic veterinary medicine for the purpose of developing the best autogenous vaccine candidates applicable to multiple aquaculture establishments.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Oxford Nanopore Technologies ; Autogenous Vaccines ; Disease ; Epidemiological Link ; Epidemiological Unit ; Furunculosis ; Infection ; Phylogeny Analysis ; Salmonids ; Third-generation Sequencing
ISSN (print) / ISBN
2076-0817
e-ISSN
2076-0817
Journal
Pathogens
Quellenangaben
Volume: 13,
Issue: 10,
Article Number: 908
Publisher
MDPI
Publishing Place
Basel
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Comparative Microbiome Analysis (COMI)