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Kriukova, E. ; Mazurenka, M. ; Marcazzan, S. ; Glasl, S. ; Quante, M.* ; Saur, D.* ; Tschurtschenthaler, M.* ; Puppels, G.J.* ; Gorpas, D. ; Ntziachristos, V.

Hybrid Raman and partial wave spectroscopy microscope for the characterization of molecular and structural alterations in tissue.

J. Biophotonics:e202400330 (2024)

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We present a hybrid Raman spectroscopy (RS) and partial wave spectroscopy (PWS) microscope for the characterization of molecular and structural tissue alterations. The PWS performance was assessed with surface roughness standards, while the Raman performance with a silicon crystal standard. We also validated the system on stomach and intestinal mouse tissues, two closely-related tissue types, and demonstrate that the addition of PWS information improves RS data classification for these tissue types from R² = 0.892 to R² = 0.964 (norm of residuals 0.863 and 0.497, respectively). Then, in a proof-of-concept experiment, we show that the hybrid system can detect changes in intestinal tissues harvested from a tumorigenic Villin-Cre, Apc^fl/wt mouse. We discuss how the hybrid modality offers new abilities to identify the relative roles of PWS morphological features and Raman molecular fingerprints, possibly allowing for their combination to enhance the study of carcinogenesis and early cancer diagnostics in the future.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Raman Spectroscopy ; Field Cancerization ; Light‐scattering Spectroscopy ; Microscopy ; Multimodal Imaging ; Partial Wave Spectroscopy; In-vivo; Field Carcinogenesis; Risk Stratification; Cancer

ISSN (print) / ISBN 1864-063X

e-ISSN 1864-0648

Journal Journal of Biophotonics

Quellenangaben Article Number: e202400330

Publisher Wiley

Publishing Place Postfach 101161, 69451 Weinheim, Germany

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Biological and Medical Imaging (IBMI)

Grants European Union
European Union's Horizon 2020 research and innovation programme