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Eckstein, Y.* ; Kessler, B.* ; Hinrichs, A.* ; Novak, I.* ; von Thaden, A.* ; Lorenzen, T.* ; Rathkolb, B. ; Scholz, A.* ; Blutke, A.* ; Koopmans, S.J.* ; Hrabě de Angelis, M. ; Christoffersen, B.* ; Wolf, E.* ; Renner, S.*

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

STAR Protoc. 6:103774 (2025)
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Open Access Green as soon as Postprint is submitted to ZB.
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Biotechnology And Bioengineering ; Metabolism ; Model Organisms; Pancreatic Beta-cells; C-peptide; Clearance; Model
Language english
Publication Year 2025
HGF-reported in Year 2025
e-ISSN 2666-1667
Journal STAR Protocols
Quellenangaben Volume: 6, Issue: 2, Pages: , Article Number: 103774 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)
POF-Topic(s) 30201 - Metabolic Health
Research field(s) Genetics and Epidemiology
PSP Element(s) G-500692-001
G-500600-001
Grants EU Innovative Health Initiative "NHPig"
Deutsche Forschungsgemeinschaft
EU FP7 Network for Initial Training (ITN) "EpiHealthNet"
German Center for Diabetes Research (DZD)
DOI 10.1016/j.xpro.2025.103774
WOS ID 001473655200001
Scopus ID 105002662027
PubMed ID 40249709
Erfassungsdatum 2025-04-29
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