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Eckstein, Y.* ; Kessler, B.* ; Hinrichs, A.* ; Novak, I.* ; von Thaden, A.* ; Lorenzen, T.* ; Rathkolb, B. ; Scholz, A.* ; Blutke, A.* ; Koopmans, S.J.* ; Hrabě de Angelis, M. ; Christoffersen, B.* ; Wolf, E.* ; Renner, S.*

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

STAR Protoc. 6:103774 (2025)

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The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.¹ and Renner et al.².

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Biotechnology And Bioengineering ; Metabolism ; Model Organisms

e-ISSN 2666-1667

Journal STAR Protocols

Quellenangaben Volume: 6, Issue: 2, Article Number: 103774

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Experimental Genetics (IEG)