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Eckstein, Y.* ; Kessler, B.* ; Hinrichs, A.* ; Novak, I.* ; von Thaden, A.* ; Lorenzen, T.* ; Rathkolb, B. ; Scholz, A.* ; Blutke, A.* ; Koopmans, S.J.* ; Hrabě de Angelis, M. ; Christoffersen, B.* ; Wolf, E.* ; Renner, S.*

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

STAR Protoc. 6:103774 (2025)
Publ. Version/Full Text DOI PMC
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Open Access Green as soon as Postprint is submitted to ZB.
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Biotechnology And Bioengineering ; Metabolism ; Model Organisms; Pancreatic Beta-cells; C-peptide; Clearance; Model
e-ISSN 2666-1667
Journal STAR Protocols
Quellenangaben Volume: 6, Issue: 2, Pages: , Article Number: 103774 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)
Grants EU Innovative Health Initiative "NHPig"
Deutsche Forschungsgemeinschaft
EU FP7 Network for Initial Training (ITN) "EpiHealthNet"
German Center for Diabetes Research (DZD)