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Gemünd, I. ; Bonaguro, L.* ; Becker, M.* ; Müller, S.* ; Joos, C. ; De Domenico, E.* ; Aschenbrenner, A.C.* ; Schultze, J.L.* ; Moosmann, A. ; Beyer, M.D.*

Characterization of human CMV-specific CD8+ T cells using multi-layer single-cell omics.

Cell Rep. Methods 5:101085 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
In this study, we established a comprehensive workflow to collect multi-omics single-cell data using a commercially available micro-well-based platform. This included whole transcriptome, cell surface markers (targeted sequencing-based cell surface proteomics), T cell specificities, adaptive immune receptor repertoire (AIRR) profiles, and sample multiplexing. With this technique, we identified paired T cell receptor sequences for three prominent human CMV epitopes. In addition, we assessed the ability of dCODE dextramers to detect antigen-specific T cells at low frequencies by estimating sensitivities and specificities when used as reagents for single-cell multi-omics.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Bd Rhapsody ; Cd8(+) T Cells ; Cmv ; Cp: Immunology ; Tcr Sequencing ; Antigen-specific T Cells ; Dcode Dextramers ; Single-cell Multi-omics ; Systems Immunology
ISSN (print) / ISBN 2667-2375
e-ISSN 2667-2375
Journal Cell Reports Methods
Quellenangaben Volume: 5, Issue: 7, Pages: , Article Number: 101085 Supplement: ,
Publisher Elsevier
Publishing Place 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Virology (VIRO)
Grants European Research Council (ERC)
European Union (EU)
BMBF-IMMME
BMBF-founded project Diet-Body-Brain (DietBB)
The iTREAT
Else Kroner-Fresenius Foundation
DFG-funded project ImmuDiet
European Research Council under the European Union's Horizon 2020 research and innovation program
German Research Foundation (DFG)