PuSH - Publication Server of Helmholtz Zentrum München

Gemünd, I. ; Bonaguro, L.* ; Becker, M.* ; Müller, S.* ; Joos, C. ; De Domenico, E.* ; Aschenbrenner, A.C.* ; Schultze, J.L.* ; Moosmann, A. ; Beyer, M.D.*

Characterization of human CMV-specific CD8+ T cells using multi-layer single-cell omics.

Cell Rep. Methods, DOI: 10.1016/j.crmeth.2025.101085:101085 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
In this study, we established a comprehensive workflow to collect multi-omics single-cell data using a commercially available micro-well-based platform. This included whole transcriptome, cell surface markers (targeted sequencing-based cell surface proteomics), T cell specificities, adaptive immune receptor repertoire (AIRR) profiles, and sample multiplexing. With this technique, we identified paired T cell receptor sequences for three prominent human CMV epitopes. In addition, we assessed the ability of dCODE dextramers to detect antigen-specific T cells at low frequencies by estimating sensitivities and specificities when used as reagents for single-cell multi-omics.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Bd Rhapsody ; Cd8(+) T Cells ; Cmv ; Cp: Immunology ; Tcr Sequencing ; Antigen-specific T Cells ; Dcode Dextramers ; Single-cell Multi-omics ; Systems Immunology
ISSN (print) / ISBN 2667-2375
e-ISSN 2667-2375
Journal Cell Reports Methods
Quellenangaben Volume: , Issue: , Pages: , Article Number: 101085 Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Virology (VIRO)