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Thaqi, S.K.* ; Hensel, N.* ; Vitow, N.* ; Baum, C.* ; Streb, L.-M. ; Kublik, S. ; Leinweber, P.* ; Panten, K.* ; Schloter, M. ; Schulz, S.

Non-rhizobial endophyte recruitment and diversity in Pisum sativum are strongly shaped by phosphorus fertilizer form.

Environ. Microbiome 20, 19:92 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
BACKGROUND: Non-rhizobial endophytes (NREs) support plant health and nodule function by enhancing symbiotic interactions and nitrogen fixation. However, their recruitment dynamics under fertilizers of varying phosphorus solubility remain poorly understood. This study investigated how four P fertilization treatments-no phosphorus (P0), bone char (BC), surface-modified bone char plus (BCplus), and triple superphosphate (TSP)-with increasing solubility influence microbial recruitment and diversity in Pisum sativum, leading to differences in plant-available phosphorus across bulk soil, rhizosphere, roots, and nodules. RESULTS: Using 16S rRNA amplicon sequencing, we found that nodule-associated microbial communities were primarily recruited from unknown sources, likely seeds, followed by roots, especially under BCplus. Phosphorus solubility of treatments significantly influenced recruitment patterns, with solubility further shaping microbial diversity. BCplus recruited beneficial taxa like Beijerinckiaceae and Flavobacteriaceae, which are associated with nitrogen fixation and biocontrol. In contrast, the highly soluble TSP treatment expanded recruitment from the rhizosphere, reflecting less stringent environmental filtering and promoting taxa like Steroidobacteraceae and Blastocatellaceae, known for nutrient cycling and pathogen suppression. In the absence of P fertilization (P0), recruitment relied heavily on seeds and roots, with arbuscular mycorrhizal fungi colonization prioritized over nodulation. Notably, TSP supported significantly more nodules with greater microbial diversity, potentially enhanced by NREs. CONCLUSIONS: Phosphorus solubility of the applied fertilizers strongly influences NRE recruitment dynamics in P. sativum. Seeds and roots act as primary reservoirs, while highly soluble fertilizers promote broader recruitment from the rhizosphere and increase microbial diversity in nodules. These results underscore the importance of the fertilization form in modulating NRE recruitment.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Pisum Sativum ; Nifh ; Amplicon ; Arbuscular Mycorrhizal Fungi ; Bone Char ; Nodulation ; Non-rhizobial Endophytes; Arbuscular Mycorrhizal Fungi; Root-nodules; Bacterial Communities; Nitrogen-fixation; Legume Evolution; Sp Nov.; Soil; Colonization; Nodulation; Abundance
ISSN (print) / ISBN 2524-6372
e-ISSN 2524-6372
Journal Environmental Microbiome
Quellenangaben Volume: 20, Issue: 1, Pages: 19, Article Number: 92 Supplement: ,
Publisher BioMed Central
Publishing Place Campus, 4 Crinan St, London N1 9xw, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)
Grants German Federal Ministry of Education and Research (BMBF)
InnoSoilPhos