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Stemberger, C.* ; Dreher, S.* ; Tschulik, C.* ; Piossek, C.* ; Bet, J.* ; Yamamoto, T.N.* ; Schiemann, M. ; Neuenhahn, M.* ; Martin, K.* ; Schlapschy, M.* ; Skerra, A.* ; Schmidt, T.* ; Edinger, M.* ; Riddell, S.R.* ; Germeroth, L.* ; Busch, D.H.

Novel serial positive enrichment technology enables clinical multiparameter cell sorting.

PLoS ONE 7:e35798 (2012)
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A general obstacle for clinical cell preparations is limited purity, which causes variability in the quality and potency of cell products and might be responsible for negative side effects due to unwanted contaminants. Highly pure populations can be obtained best using positive selection techniques. However, in many cases target cell populations need to be segregated from other cells by combinations of multiple markers, which is still difficult to achieve - especially for clinical cell products. Therefore, we have generated low-affinity antibody-derived Fab-fragments, which stain like parental antibodies when multimerized via Strep-tag and Strep-Tactin, but can subsequently be removed entirely from the target cell population. Such reagents can be generated for virtually any antigen and can be used for sequential positive enrichment steps via paramagnetic beads. First protocols for multiparameter enrichment of two clinically relevant cell populations, CD4(high)/CD25(high)/CD45RA(high) 'regulatory T cells' and CD8(high)/CD62L(high)/CD45RA(neg) 'central memory T cells', have been established to determine quality and efficacy parameters of this novel technology, which should have broad applicability for clinical cell sorting as well as basic research.
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Publication type Article: Journal article
Document type Scientific Article
Keywords VERSUS-HOST-DISEASE; ONE-STEP PURIFICATION; REGULATORY T-CELLS; F-AB FRAGMENT; ADOPTIVE TRANSFER; CRYSTAL-STRUCTURE; ANTIBODY; LYMPHOCYTES; TRANSPLANTATION; IMMUNOTHERAPY
Language english
Publication Year 2012
HGF-reported in Year 2012
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 7, Issue: 4, Pages: , Article Number: e35798 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Reviewing status Peer reviewed
Institute(s) Institute of Virology (VIRO)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Immune Response and Infection
PSP Element(s) G-520100-001
PubMed ID 22545138
DOI 10.1371/journal.pone.0035798
WOS ID WOS:000305343200058
Scopus ID 84860169849
Erfassungsdatum 2012-07-12
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