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Koch, M. ; de Jong, J.S.* ; Glatz, J. ; Symvoulidis, P. ; Lamberts, L.E.* ; Adams, A.L.L.* ; Kranendonk, M.E.G.* ; Terwisscha van Scheltinga, A.G.* ; Aichler, M. ; Jansen, L.* ; de Vries, J.* ; Lub-de, Hoog, M.N.* ; Schröder, C.P.* ; Jorritsma-Smit, A.* ; Linssen, M.D.* ; de Boer, E.* ; van der Vegt, B.* ; Nagengast, W.B.* ; Elisas,S.G.* ; Oliveira, S.* ; Witkamp, A.J.* ; Mali, W.P.Th.M.* ; van der Wall, E.* ; Gracia-Allende, P.B. ; van Diest, P.J.* ; de Vries, E.G.* ; Walch, A.K. ; van Dam, G.M.* ; Ntziachristos, V.

Threshold analysis and biodistribution of fluorescently labeled bevacizumab in human breast cancer.

Cancer Res. 77, 623-631 (2017)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
In vivo tumor labeling with fluorescent agents may assist endoscopic and surgical guidance for cancer therapy as well as create opportunities to directly observe cancer biology in patients. However, malignant and non-malignant tissues are usually distinguished on fluorescence images by applying empirically determined fluorescence intensity thresholds. Here we report the development of fSTREAM, a set of analytic methods designed to streamline the analysis of surgically excised breast tissues by collecting and statistically processing hybrid multi-scale fluorescence, color, and histology readouts toward precision fluorescence imaging. fSTREAM addresses core questions of how to relate fluorescence intensity to tumor tissue and how to quantitatively assign a normalized threshold that sufficiently differentiates tumor tissue from healthy tissue. Using fSTREAM we assessed human breast tumors stained in vivo with fluorescent bevacizumab at microdose levels Showing that detection of such levels is achievable, we validated fSTREAM for high-resolution mapping of the spatial pattern of labeled antibody and its relation to the underlying cancer pathophysiology and tumor border on a per patient basis. We demonstrated a 98% sensitivity and 79% specificity when using labelled bevacizumab to outline the tumor mass. Overall, our results illustrate a quantitative approach to relate fluorescence signals to malignant tissues and improve the theranostic application of fluorescence molecular imaging.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Indocyanine Green; Folate; Surgery; Margins; Safety; Agent; Head
Sprache englisch
Veröffentlichungsjahr 2017
Prepublished im Jahr 2016
HGF-Berichtsjahr 2016
ISSN (print) / ISBN 0008-5472
e-ISSN 1538-7445
Zeitschrift Cancer Research
Quellenangaben Band: 77, Heft: 3, Seiten: 623-631 Artikelnummer: , Supplement: ,
Verlag American Association for Cancer Research (AACR)
Verlagsort Philadelphia, Pa.
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Research Unit Analytical Pathology (AAP)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505500-001
G-500390-001
DOI 10.1158/0008-5472.CAN-16-1773
WOS ID WOS:000393194400005
Scopus ID 85012891746
PubMed ID 27879266
Erfassungsdatum 2016-11-25
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