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Breunig, M.* ; Merkle, J.* ; Melzer, M.K.* ; Heller, S.* ; Seufferlein, T.* ; Meier, M. ; Hohwieler, M.* ; Kleger, A.*

Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.

STAR Protoc. 2:100913 (2021)

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The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive. For complete details on the use and execution of this protocol, please refer to Breunig et al. (2021).

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