PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Marcazzan, S. ; Braz Carvalho, M.J.* ; Konrad, M.* ; Strangmann, J.* ; Tenditnaya, A. ; Baumeister, T.* ; Schmid, R.M.* ; Wester, H.J.* ; Ntziachristos, V. ; Gorpas, D. ; Wang, T.C.* ; Schottelius, M.* ; Quante, M.*

CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett's esophagus.

EJNMMI Res. 12:2 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
BACKGROUND: Near-infrared (NIR) fluorescence imaging has been emerging as a promising strategy to overcome the high number of early esophageal adenocarcinomas missed by white light endoscopy and random biopsy collection. We performed a preclinical assessment of fluorescence imaging and endoscopy using a novel CXCR4-targeted fluorescent peptide ligand in the L2-IL1B mouse model of Barrett's esophagus. METHODS: Six L2-IL1B mice with advanced stage of disease (12-16 months old) were injected with the CXCR4-targeted, Sulfo-Cy5-labeled peptide (MK007), and ex vivo wide-field imaging of the whole stomach was performed 4 h after injection. Before ex vivo imaging, fluorescence endoscopy was performed in three L2-IL1B mice (12-14 months old)  by a novel imaging system with two L2-IL1B mice used as negative controls. RESULTS: Ex vivo imaging and endoscopy in L2-IL1B mice showed that the CXCR4-targeted MK007 accumulated mostly in the dysplastic lesions with a mean target-to-background ratio > 2. The detection of the Sulfo-Cy5 signal in dysplastic lesions and its co-localization with CXCR4 stained cells  by confocal microscopy further confirmed the imaging results. CONCLUSIONS: This preliminary preclinical study shows that CXCR4-targeted fluorescence endoscopy using MK007 can detect dysplastic lesions in a mouse model of Barrett's esophagus. Further investigations are needed to assess its use in the clinical setting.
Impact Factor
Scopus SNIP
Altmetric
3.434
1.065
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Animal Models ; Barrett’s Esophagus ; Cxcr4 ; Dysplasia ; Endoscopy ; Esophageal Cancer ; Fluorescence Imaging ; Molecular Imaging ; Peptide; Cancer; Expression; Pet
Sprache englisch
Veröffentlichungsjahr 2022
HGF-Berichtsjahr 2022
ISSN (print) / ISBN 2191-219X
e-ISSN 2191-219X
Zeitschrift EJNMMI Research
Quellenangaben Band: 12, Heft: 1, Seiten: , Artikelnummer: 2 Supplement: ,
Verlag Springer
Verlagsort One New York Plaza, Suite 4600, New York, Ny, United States
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505500-001
Förderungen Technische Universität München
Deutsche Forschungsgemeinschaft
DOI 10.1186/s13550-021-00875-7
WOS ID WOS:000740997400002
Scopus ID 85119688860
PubMed ID 35006394
Erfassungsdatum 2022-05-30
[➜Korrektur melden]