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Zimmermann, S.* ; Mathew, A.* ; Bondareva, O. ; Elwakiel, A.* ; Jiang, S.* ; Rana, R.* ; Bechmann, I.* ; Goldschmidt, J.* ; Klöting, N. ; Sheikh, B. ; Isermann, B.*

Noncanonical microglial IL-1β maturation in chronic kidney disease.

Nephrol. Dial. Transplant. 40, 929-942 (2024)
Postprint DOI PMC
Open Access Green
BACKGROUND AND HYPOTHESIS: Organ transplantation reverses cognitive impairment in chronic kidney disease (CKD), indicating that cognitive impairment driven by CKD is therapeutically amendable. We recently demonstrated that impaired cognition in CKD is linked to IL-1β-release from microglia and IL-1R1-signaling in neuronal cells, thereby identifying a signaling pathway that can be exploited therapeutically. However, the mechanism of IL-1β-maturation in microglia in CKD remains unknown. We hypothesized that microglia cells require caspase-1 for CKD-driven cognitive impairment. METHODS: We used a combination of single cell analyses, in situ analyses, genetically modified mouse models (including newly generated Cre-LoxP mouse models) and in vitro models. The current study builds on a recently identified intercellular crosstalk between microglia and neurons that impairs cognition in chronic kidney disease (CKD). RESULTS: Here, we show that despite NLRP3 inflammasome activation in the brain and protection of mice with constitutive NLRP3 deficiency from CKD-induced cognitive impairment, (i) caspase-1 is not required for IL-1β maturation in microglia and (ii) targeted caspase-1 deficiency in microglia does not improve cognition in CKD mice. These data indicate that IL-1β maturation in microglia is independent of the NLRP3-caspase-1 interaction in CKD. Indeed, microglia activation in CKD induces noncanonical, cathepsin C-caspase-8 mediated IL-1β maturation. Depletion of cathepsin C or caspase-8 blocks IL-1β maturation in microglia. Preliminary analyses suggest that noncanonical microglia IL-1β maturation occurs also in diabetes mellitus. CONCLUSION: These results identify a noncanonical IL-1β-maturation pathway as a potential therapeutic target to combat microglia-induced neuronal dysfunction in CKD and possible other peripheral diseases.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Caspase-8 ; Cathepsins ; Chronic Diseases ; Cognition ; Inflammation ; Microglia Signaling; Diabetic-nephropathy; Nlrp3 Inflammasome; Oxidative Stress; Activation; Pathology; Cirrhosis; Pathway; Injury
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 0931-0509
e-ISSN 1460-2385
Zeitschrift Nephrology Dialysis Transplantation
Quellenangaben Band: 40, Heft: 5, Seiten: 929-942 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Great Clarendon St, Oxford Ox2 6dp, England
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
POF Topic(s) 30201 - Metabolic Health
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-506502-001
G-506500-001
G-555000-001
Förderungen Medical Faculty of the University of Leipzig
Deutscher Akademischer Austauschdienst
DFG
German Research Foundation)
Deutsche Forschungsgemeinschaft (DFG
DOI 10.1093/ndt/gfae239
WOS ID 001367210000001
Scopus ID 105003890296
PubMed ID 39496522
Erfassungsdatum 2024-11-06
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