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Stemberger, C.* ; Dreher, S.* ; Tschulik, C.* ; Piossek, C.* ; Bet, J.* ; Yamamoto, T.N.* ; Schiemann, M. ; Neuenhahn, M.* ; Martin, K.* ; Schlapschy, M.* ; Skerra, A.* ; Schmidt, T.* ; Edinger, M.* ; Riddell, S.R.* ; Germeroth, L.* ; Busch, D.H.

Novel serial positive enrichment technology enables clinical multiparameter cell sorting.

PLoS ONE 7:e35798 (2012)
Verlagsversion Volltext DOI PMC
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A general obstacle for clinical cell preparations is limited purity, which causes variability in the quality and potency of cell products and might be responsible for negative side effects due to unwanted contaminants. Highly pure populations can be obtained best using positive selection techniques. However, in many cases target cell populations need to be segregated from other cells by combinations of multiple markers, which is still difficult to achieve - especially for clinical cell products. Therefore, we have generated low-affinity antibody-derived Fab-fragments, which stain like parental antibodies when multimerized via Strep-tag and Strep-Tactin, but can subsequently be removed entirely from the target cell population. Such reagents can be generated for virtually any antigen and can be used for sequential positive enrichment steps via paramagnetic beads. First protocols for multiparameter enrichment of two clinically relevant cell populations, CD4(high)/CD25(high)/CD45RA(high) 'regulatory T cells' and CD8(high)/CD62L(high)/CD45RA(neg) 'central memory T cells', have been established to determine quality and efficacy parameters of this novel technology, which should have broad applicability for clinical cell sorting as well as basic research.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter VERSUS-HOST-DISEASE; ONE-STEP PURIFICATION; REGULATORY T-CELLS; F-AB FRAGMENT; ADOPTIVE TRANSFER; CRYSTAL-STRUCTURE; ANTIBODY; LYMPHOCYTES; TRANSPLANTATION; IMMUNOTHERAPY
Sprache englisch
Veröffentlichungsjahr 2012
HGF-Berichtsjahr 2012
ISSN (print) / ISBN 1932-6203
Zeitschrift PLoS ONE
Quellenangaben Band: 7, Heft: 4, Seiten: , Artikelnummer: e35798 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort Lawrence, Kan.
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-520100-001
PubMed ID 22545138
DOI 10.1371/journal.pone.0035798
WOS ID WOS:000305343200058
Scopus ID 84860169849
Erfassungsdatum 2012-07-12
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