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Meinhold, M.* ; Verbrugge, S.* ; Shi, A.* ; Schönfelder, M.* ; Becker, L. ; Jaspers, R.T.* ; Zammit, P.S.* ; Wackerhage, H.*

Yap/Taz activity is associated with increased expression of phosphoglycerate dehydrogenase that supports myoblast proliferation.

Cell Tissue Res. 395, 271–283 (2024)

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In skeletal muscle, the Hippo effector Yap promotes satellite cell, myoblast, and rhabdomyoblast proliferation but prevents myogenic differentiation into multinucleated muscle fibres. We previously noted that Yap drives expression of the first enzyme of the serine biosynthesis pathway, phosphoglycerate dehydrogenase (Phgdh). Here, we examined the regulation and function of Phgdh in satellite cells and myoblasts and found that Phgdh protein increased during satellite cell activation. Analysis of published data reveal that Phgdh mRNA in mouse tibialis anterior muscle was highly expressed at day 3 of regeneration after cardiotoxin injection, when markers of proliferation are also robustly expressed and in the first week of synergist-ablated muscle. Finally, siRNA-mediated knockdown of PHGDH significantly reduced myoblast numbers and the proliferation rate. Collectively, our data suggest that Phgdh is a proliferation-enhancing metabolic enzyme that is induced when quiescent satellite cells become activated.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Myoblast ; Phgdh ; Phosphoglycerate Dehydrogenase ; Skeletal Muscle ; Taz ; Yap; Skeletal-muscle; Gene-expression; Satellite Cells; 3-phosphoglycerate Dehydrogenase; Serine Synthesis; Hippo Pathway; Reveals; Yap; Metabolism; Drosophila

ISSN (print) / ISBN 0044-3794

e-ISSN 1432-0878

Journal Cell and Tissue Research

Quellenangaben Volume: 395, Pages: 271–283

Publisher Springer

Publishing Place One New York Plaza, Suite 4600, New York, Ny, United States

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Experimental Genetics (IEG)

Grants Technische Universitt Mnchen (1025)