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Stiel, A.-C. ; Ntziachristos, V.

Controlling the sound of light: photoswitching optoacoustic imaging.

Nat. Methods, DOI: 10.1038/s41592-024-02396-2 (2024)
DOI PMC
Open Access Green: Postprint online available 04/2025
Optoacoustic (photoacoustic) imaging advances allow high-resolution optical imaging much deeper than optical microscopy. However, while label-free optoacoustics have already entered clinical application, biological imaging is in need of ubiquitous optoacoustic labels for use in ways that are similar to how fluorescent proteins propelled optical microscopy. We review photoswitching advances that shine a new light or, in analogy, 'bring a new sound' to biological optoacoustic imaging. Based on engineered labels and novel devices, switching uses light or other energy forms and enables signal modulation and synchronous detection for maximizing contrast and detection sensitivity over other optoacoustic labels. Herein, we explain contrast enhancement in the spectral versus temporal domains and review labels and key concepts of switching and their properties to modulate optoacoustic signals. We further outline systems and applications and discuss how switching can enable optoacoustic imaging of cellular or molecular contrast at depths and resolutions beyond those of other optical methods.
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Publication type Article: Journal article
Document type Review
Corresponding Author
Keywords Infrared Fluorescent Proteins; Deep Tissue; Tomography; Microscopy; Mesoscopy
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Journal Nature Methods
Publisher Nature Publishing Group
Publishing Place New York, NY
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Grants European Research Council (ERC)
Deutsche Forschungsgemeinschaft
European Union